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The expansion of fluorescence bioimaging toward more complex systems and geometries requires analytical tools capable of spanning widely varying timescales and length scales, cleanly separating multiple fluorescent labels and distinguishing these labels from background autofluorescence. Here we meet these challenging objectives for multispectral fluorescence microscopy, combining hyperspectral phasors and linear unmixing to create Hybrid Unmixing (HyU). HyU is efficient and robust, capable of quantitative signal separation even at low illumination levels. In dynamic imaging of developing zebrafish embryos and in mouse tissue, HyU was able to cleanly and efficiently unmix multiple fluorescent labels, even in demanding volumetric timelapse imaging settings. HyU permits high dynamic range imaging, allowing simultaneous imaging of bright exogenous labels and dim endogenous labels. This enables coincident studies of tagged components, cellular behaviors and cellular metabolism within the same specimen, providing more accurate insights into the orchestrated complexity of biological systems.

 

We present a drift kinetic model for the free expansion of a thermal plasma out of a magnetic nozzle. This problem relates to plasma space propulsion systems, natural environments such as the solar wind, and end losses from the expander region of mirror magnetically confined fusion concepts such as the gas dynamic trap. The model incorporates trapped and passing orbit types encountered in the mirror expander geometry and maps to an upstream thermal distribution. This boundary condition and quasineutrality require the generation of an ambipolar potential drop of 5Te=e, forming a thermal barrier for the electrons. The model for the electron and ion velocity distributions and fluid moments is confirmed with data from a fully kinetic simulation. Finally, the model is extended to account for a population of fast sloshing ions arising from neutral beam heating within a magnetic mirror, again resulting in good agreement with a corresponding kinetic simulation. 

The orientation and stability of the reconnection x line in asymmetric geometry is studied using three‐dimensional (3‐D) particle‐in‐cell simulations. We initiate reconnection at the center of a large simulation domain to minimize the boundary effect. The resulting x line has sufficient freedom to develop along an optimal orientation, and it remains laminar. Companion 2‐D simulations indicate that this x line orientation maximizes the reconnection rate. The divergence of the nongyrotropic pressure tensor breaks the frozen‐in condition, consistent with its 2‐D counterpart. We then design 3‐D simulations with one dimension being short to fix the x line orientation but long enough to allow the growth of the fastest growing oblique tearing modes. This numerical experiment suggests that reconnection tends to radiate secondary oblique tearing modes if it is externally (globally) forced to proceed along an orientation not favored by the local physics. The development of oblique structure easily leads to turbulence inside small periodic systems. 

Lower‐hybrid‐drift waves driving vortical flows have recently been discovered in the electron current layer during magnetic reconnection in the terrestrial magnetotail. Yet, spacecraft measurements cannot address how pervasive the waves are. We perform three‐dimensional particle‐in‐cell simulations of guide field reconnection to demonstrate that electron vortices driven by the lower‐hybrid‐drift instability (LHDI) are excited immediately downstream from the electron jet reversal in 3‐D channels of enhanced electron outflow. The resulting fluctuations generate a series of alternating vortices, producing magnetic field perturbations opposing and enhancing the local guide field and causing kinking of the enhanced electron outflow and patches of increased current. Our results demonstrate for the first time that LHDI exists in the electron current layer and enhanced outflow channels, providing a conceptual breakthrough on the LHDI in reconnection.